Time course proteomic profile of rat acute myocardial infarction by SELDI-TOF MS analysis

Zhang, Gengqian; Zhou, Bin; Zheng, Yanhua; Feng, Kai; Rao, Li; Zhang, Jianzhong; Xin, Junping; Zhang, Beilei; Zhang, Lin

doi:10.1016/j.ijcard.2007.10.021

« Previous Next »International Journal of Cardiology
Volume 131, Issue 2 , Pages 225-233, 9 January 2009

Time course proteomic profile of rat acute myocardial infarction by SELDI-TOF MS analysis☆

Gengqian Zhang
- Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, PR China
- Now working at School of Forensic Medicine, Shanxi Medical University, Taiyuan, 030001, PR China.
Bin Zhou
- Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, PR China
- Now working at Laboratory of Molecular Translational Medicine, West China Second University Hospital, Sichuan University, Chengdu 610041, PR China.
Yanhua Zheng
- Department of Pathology and Laboratory Medicine, Beijing 306 Hospital, Beijing, 100101, PR China
Kai Feng
- Department of Pathology and Laboratory Medicine, Beijing 306 Hospital, Beijing, 100101, PR China
Li Rao
- Department of Cardiology, West China Hospital, Sichuan University, Chengdu, 610041, PR China
Jianzhong Zhang
- Department of Pathology and Laboratory Medicine, Beijing 306 Hospital, Beijing, 100101, PR China
Junping Xin
- Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, PR China
- Currently studying in Department of Cell Biology, Neurobiology and Anatomy, Loyola University Medical Center, 2160 S First Avenue, Maywood, IL, 60153, USA
Beilei Zhang
- Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, PR China
Lin Zhang
- Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, PR China
- State Key Laboratory of Biotherapy of Human Diseases, West China Medical School, Sichuan University, Sichuan, 610041, PR China
- Corresponding author. Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Number 17, Section 3, South Renmin Road, Chengdu, 610041, PR China. Tel.: +86 28 85469033; fax: +86 28 85401825.

Received 22 February 2007; received in revised form 30 August 2007; accepted 20 October 2007. published online 07 January 2008.

Abstract

Background

Surface-enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF MS) is a powerful tool to detect biomarkers of many diseases. The purpose of this study is to evaluate SELDI-TOF MS as a potential tool for identifying serological biomarkers of acute myocardial infarction (AMI) at an early stage.

Methods

Serum samples were collected from rats after they had received left anterior descending coronary artery (LAD) ligation at 5, 15, 30, 60, 120, 240 and 360 min, respectively. These samples, along with serum samples from the sham-operated and uninjured control animals, were processed and analyzed with CM-10 ProteinChip and SELDI-TOF MS. Levels of myocardial creatine kinase isoenzyme MB (CKMB) activity and cardiac troponin I (cTnI) concentration were measured with respective commercial kits. Histological changes were examined after HE staining.

Results

Three peaks with m/z 7586 Da, 7564 Da and 9583 Da, which were only found in the operated groups, were termed specific peaks. The peaks with m/z 7586 and 7564 Da had the sensitivity of 97.0% and specificity of 97.0% in the ≥15 min operated groups. Four peaks with m/z 4983, 5140, 8075, and 9423 Da were novel tissue unspecific injury related peaks in response to injury. Four peaks with m/z 4400, 4542, 8400 and 7666 Da exhibited more than 2 fold peak height alterations. In contrast, the significant increase of CKMB activity and cTnI concentration in the serum could not be detected until 4 h after the ligation.

Conclusions

The specific peaks and unspecific injury related peaks detected by SELDI-TOF MS with CM-10 ProteinChip may be used as the biomarkers for early diagnosis of AMI.

Abbreviations: AMI, acute myocardial infarction, CKMB, creatine kinase isoenzyme MB, cTnI, cardiac troponin I, ECG, electrocardiogram, ELISA, enzyme-linked immunosorbent assay, LAD, left anterior descending coronary artery, m/z, mass-to-charge ratio, SCD, sudden cardiac death, SELDI-TOF MS, Surface-enhanced laser desorption/ionization time of flight mass spectrometry.