International Journal of Cardiology
Volume 134, Issue 1 , Pages 75-81 , 1 May 2009

Cardiac differentiation of P19CL6 cells by oxytocin

  • Fardin Fathi

      Affiliations

    • KDRC, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
    • Corresponding Author InformationCorresponding author. Tel.: +98 871 6661830; fax: +98 871 6664674.
    • ,
  • Satoshi Murasawa

      Affiliations

    • Group of Vascular Regeneration Research, Kobe Institute of Biomedical Research and Innovation/Riken Center for Developmental Biology, 2-2 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Japan
    • ,
  • Satoshi Hasegawa

      Affiliations

    • Group of Vascular Regeneration Research, Kobe Institute of Biomedical Research and Innovation/Riken Center for Developmental Biology, 2-2 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Japan
    • ,
  • Takayuki Asahara

      Affiliations

    • Group of Vascular Regeneration Research, Kobe Institute of Biomedical Research and Innovation/Riken Center for Developmental Biology, 2-2 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Japan
    • ,
  • Abbas Jafari Kermani

      Affiliations

    • Department of Genetics, Faculty of Basic Sciences, Tarbiat Modares University, Tehran, Iran
    • ,
  • Seyed Javad Mowla

      Affiliations

    • Department of Genetics, Faculty of Basic Sciences, Tarbiat Modares University, Tehran, Iran

Received 9 May 2007 ,Revised 31 August 2007 ,Accepted 14 January 2008.

References 

  1. McBurney MW, Jones-Villeneuve EMV, Edwards MKS, Anderson PJ. Control of muscle and neuronal differentiation in a cultured embryonal carcinoma cell line. Nature. 1982;299:165–167
  2. McBurney MW. P19 embryonal carcinoma cells. Int J Dev Biol. 1993;37:135–140
  3. Paquin J, Danalache BA, Jankowski M, et al. Oxytocin induces differentiation of P19 embryonic stem cells to cardiomyocytes. Proc Natl Acad Sci USA. 2002;99:9550–9555
  4. Skerjanc IS. Cardiac and skeletal muscle development in P19 embryonal carcinoma cells. Trends Cardiovasc Med. 1999;9:139–143
  5. Habara-Ohkubo A. Differentiation of beating cardiac muscle cells from a derivative of P19 embryonal carcinoma cells. Cell Struct Funct. 1996;21:101–110
  6. Van der Heyden MA, Defize LH. Twenty one years of P19 cells: what an embryonal carcinoma cell line taught us about cardiomyocyte differentiation. Cardiovasc Res. 2003;58:292–302
  7. Monzen K, Hiroi Y, Kudoh S, et al. Smads, TAK1, and their common target ATF-2 play a critical role in cardiomyocyte differentiation. J Cell Biol. 2001;153(4):687–698
  8. Gimpl G, Fahrenholz F. The oxytocin receptor system: structure, function and regulation. Physiol Rev. 2001;81:629–683
  9. Gutkowska J, Jankowski M, Lambert C, et al. Oxytocin releases atrial natriuretic peptide by combining with oxytocin receptors in the heart. Proc Natl Acad Sci USA. 1997;94:11704–11709
  10. Jankowski M, Hajjar F, Al Kawas S, et al. Rat heart: a site of oxytocin production and action. Proc Natl Acad Sci USA. 1998;95:14558–14563
  11. Matsuura K, Nagai T, Nishigaki N, et al. Adult cardiac sca-1-positive cells differentiate into beating cardiomyocytes. J Biol Chem. 2004;279(12):11384–11391
  12. Hatami L, Valojerdi MR, Mowla SJ. Effects of oxytocin on cardiomyocyte differentiation from mouse embryonic stem cells. Int J Cardiol. Apr 12 2007;117(1):80–89
  13. Nemir M, Croquelois A, Pedrazzini T, et al. Induction of cardiogenesis in embryonic stem cells via downregulation of Notch1 signaling. Circ Res. 2006;98(12):1471–1478
  14. Xu W, Zhang X, Qian H, et al. Mesenchymal stem cells from adult human bone marrow differentiate into a cardiomyocyte phenotype in vitro. Exp Biol Med (Maywood). 2004;229:623–631
  15. Kadivar M, Khatami S, Mortazavi Y. In vitro cardiomyogenic potential of human umbilical vein-derived mesenchymal stem cells. Biochem Biophys Res Commun. 2006;340:639–647
  16. Makino S, Fukuda K, Miyoshi S. Cardiomyocytes can be generated from marrow stromal cells in vitro. J Clin Invest. 1999;103:697–705
  17. Mummery C, Ward-van Oostwaard D, Doevendans P, et al. Differentiation of human embryonic stem cells to cardiomyocytes: role of coculture with visceral endoderm-like cells. Circulation. 2003;107:2733–2740
  18. Moore JC, Spijker R, Martens AC. A P19Cl6 GFP reporter line to quantify cardiomyocyte differentiation of stem cells. Int J Dev Biol. 2004;48:47–55
  19. Skerjanc IS, Petropoulos H, Ridgeway AG, Wilton S. Myocyte enhancer 2C and Nkx2-5 up-regulate each other's expression and initiate cardiomyogenesis in P19 cells. J Biol Chem. 1998;52:34904–34910
  20. Jamali M, Karamboulas C, Rogerson PJ, Skerjanc IS. BMP signaling regulates Nkx2-5 activity during cardiomyogenesis. FEBS Lett. 2001;509:126–130
  21. Hosoda T, Monzen K, Hiroi Y, et al. A novel myocyte-specific gene mirori promotes the differentiation of P19CL6 cells into cardiomyocytes. J Biol Chem. 2001;276:35978–35989

PII: S0167-5273(08)00341-0

doi: 10.1016/j.ijcard.2008.01.046

International Journal of Cardiology
Volume 134, Issue 1 , Pages 75-81 , 1 May 2009

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