Improvement of cardiac function and remodeling by transplanting adipose tissue-derived stromal cells into a mouse model of acute myocardial infarction

Yu, Long Hao; Kim, Moo Hyun; Park, Tae Ho; Cha, Kwang Soo; Kim, Young Dae; Quan, Mei Lian; Rho, Mi Sook; Seo, Su Yeoung; Jung, Jin Sup

doi:10.1016/j.ijcard.2008.10.024

« Previous Next »International Journal of Cardiology
Volume 139, Issue 2 , Pages 166-172, 4 March 2010

Improvement of cardiac function and remodeling by transplanting adipose tissue-derived stromal cells into a mouse model of acute myocardial infarction

Long Hao Yu
- Department of Cardiology, College of Medicine, Dong-A University, Busan, South Korea
Moo Hyun Kim
- Department of Cardiology, College of Medicine, Dong-A University, Busan, South Korea
- Corresponding author. Department of Cardiology, Dong-A University College of Medicine, 3-1, Dongdaesin-dong, Seogu, Busan, South Korea 602-715. Tel.: +82 51 240 2976; fax: +82 51 243 0116.
Tae Ho Park
- Department of Cardiology, College of Medicine, Dong-A University, Busan, South Korea
Kwang Soo Cha
- Department of Cardiology, College of Medicine, Dong-A University, Busan, South Korea
Young Dae Kim
- Department of Cardiology, College of Medicine, Dong-A University, Busan, South Korea
Mei Lian Quan
- Department of Pathology, College of Medicine Dong-A University, Busan, South Korea
Mi Sook Rho
- Department of Pathology, College of Medicine Dong-A University, Busan, South Korea
Su Yeoung Seo
- Department of Microbiology, College of Medicine, Dong-A University, Busan, South Korea
Jin Sup Jung
- Department of Physiology, College of Medicine, Pusan National University, Busan, South Korea

Received 18 February 2008; received in revised form 24 July 2008; accepted 12 October 2008. published online 01 December 2008.

Abstract

Background

We investigated the effect of adipose tissue-derived stromal cells (ADSC) therapy on cardiac contractility and remodeling in the C57BL/6 mouse model of acute myocardial infarction (AMI).

Methods

30 adult male C57BL/6 mice were randomized into 2 groups, namely, AMI+media (control, n=15) and AMI+ADSC (n=15). AMI was produced by left anterior descending coronary artery ligation. After AMI induction, 1×10⁶ ADSC or media were intramyocardially injected and the results compared. Echocardiographic and histological analyses of surviving mice (n=20) were conducted. Echocardiography was performed before cell implantation and 2 weeks after transplantation.

Results

LVEF and FS improved in the ADSC group compared to the control (P<0.01). LVEDD in the ADSC group decreased slightly from 4.65±0.63 mm to 4.14±0.53 mm compared to the control, but there was no statistical difference (P=0.072). LVESD decreased significantly in the ADSC group (P<0.05). A significant difference in scar formation and infarct size was observed between the ADSC and control group 2 weeks after AMI (P<0.05). ADSC were observed to migrate into injured sites and integrate into scar areas and increased vascular density in the infarct site compared to control group (P<0.05). Additionally, some transplanted ADSC expressed the endothelial marker.

Conclusions

Echocardiography and histological analysis revealed that improvement in cardiac function and ventricular remodeling was better in the ADSC group than in the control. This suggests that ADSC is a good candidate for cell therapy in cardiovascular disease.

Keywords: Myocardial infarction, Adipose tissue, Transplantation