Abstract
Low energy level irradiation (LLLI) has been found to modulate biological processes.
The effect of LLLI on the development of acute myocardial infarction (MI) was investigated
following chronic ligation of the left anterior descending (LAD) coronary artery in
laboratory rats. The hearts of 22 rats were laser irradiated (LI) using a diode laser
(804 nm, 38 mW power output) through the intercostal muscles in the chest following
MI and on day 3 post MI. In the control non laser irradiated (NLI) group (19 rats)
MI was induced experimentally and laser irradiation was not applied. All rats were
sacrificed 21 days post MI. Size, thickness and relative circumferential length of
the infarct, as well as other parameters, were determined from histological sections
stained with Masson’s trichrome and hearts stained with triphenyl tetrazolium chloride
(TTC) using histomorphometric methods. The infarct size (expressed as percent of total
left ventricle area) of the LI rats was 10.1±5.8, which was significantly lower (65%;
P<0.01) than the infarct size of NLI rats which was 28.7±9.6. Correlatively, the ratio
of circumferential length of the infarcted area was significantly lower (2-fold; P<0.01) in the LI rats as compared to the NLI rats. LLLI of the infarcted area in the
myocardium of experimentally induced MI rats, at the correct energy, duration and
timing, markedly reduces the loss of myocardial tissue. This phenomenon may have an
important beneficial effect on patients after acute MI or ischemic heart disease.
Keywords
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Article info
Publication history
Accepted:
March 13,
2001
Received in revised form:
March 9,
2001
Received:
December 13,
2000
Identification
Copyright
© 2001 Elsevier Science B.V. Published by Elsevier Inc. All rights reserved.